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Download 3-Folds in P5 of degree 12of degree 12 by Edelmann G. PDF

By Edelmann G.

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Lyophilization of tissues can be another option, but its influence on the stability has to be checked [19]. At the time of analysis, whole organs (spleen, kidney, testes) or parts of an organ (liver) are first cut into small pieces. 1). This simple instrument is made of a glass vessel (like a cold-finger) and a Teflon pestle of slightly smaller diameter. Tissue and some buffer are placed in the glass receptacle. By moving the Teflon pestle up and down in the glass vessel the tissue cells are crushed between the pestle and the glass wall.

So, speciation of metal species in serum samples is to be preferred to speciation in plasma samples. ETS tubes with a red stopper contain no additives (Vacutainer , Becton Dickinson), while tubes with a green or lavender stopper contain respectively heparin and EDTA. The use of tubes without additives is strongly recommended. g. for clinical analyses. The blood is allowed to clot spontaneously and the samples are transported to a clean laboratory. After clotting, the serum is separated from the erythrocytes by centrifugation at 2500 rpm for 20 min.

The equipment is then dried upside down on a Teflon foil or polypropylene tray at 55 ◦ C in an especially reserved oven. Afterwards, the instruments are stored in an airtight plastic transport container until use. All steps are carried out in clean laboratory conditions (class 100) except boiling for 8 h in the 1 : 1 mixture of concentrated acids. Other high purity items are cleaned in a similar way. , when the items are too small, they are boiled for an additional 6–8 h in Milli-Q water. 5 % HNO3 or 1 % EDTA with a final rinse in distilled water [6].

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