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Download Atlas of human sperm morphology evaluation by T F Kruger; Daniel R Franken PDF

By T F Kruger; Daniel R Franken

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Penetration of human spermatozoa into the human zona pellucida and the zonafree hamster egg: a study of fertile donors and infertile patients.  In Keel BA, Webster BW, eds.  Characterization of the biologic activities of a recombinant human zona pellucida protein 3 expressed in human ovarian teratocarcinoma (PA­1) cells.  Outcome of intracytoplasmic sperm injection in azoospermic patients: stressing the liaison between the urologist and reproductive medicine specialist. 11 showed the AI to be a more sensitive parameter, compared with normal sperm morphology evaluated according to strict criteria, in the prediction of expected in vitro fertilization rates of ≥50%; the use of the AI was found to be particularly useful in the P­ pattern group of patients.

Male infertility problems may be successfully treated with (a) urological interventions (surgical or non­surgical treatments, such as conventional, microsurgical, or laparoscopic surgery, including correction of varicocele, epididymo­ and vaso­vasostomy and modern approaches for ejaculatory disorders); (b) medical therapies (such as specific treatment of hypogonadism, hyperprolactinemia, and infection); while (c) a significant proportion will proceed to low­ and high­complexity ARTs (assisted­reproduction techniques).

The ability for zona pellucida­bound spermatozoa to undergo the zona pellucida­induced acrosome reaction may also play an important role in non­fertilization18,19.  With good bright­field optics, at 1000× or preferably 1250× magnification and good Papanicolaou staining of the spermatozoa, valuable information on acrosomal morphology can be obtained1,11.  This means that the acrosome index will always be equal, but in most cases greater, compared with the percentage of morphologically normal spermatozoa.

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