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Download Biological Membranes: Structure, Biogenesis and Dynamics by William Dowhan (auth.), Jos A. F. Op den Kamp (eds.) PDF

By William Dowhan (auth.), Jos A. F. Op den Kamp (eds.)

The complicated research Institute on "Structure, Biogenesis and Dynamics of organic Membranes, held in Cargese from June 14-26, 1993, has been facing 4 significant themes in membrane biochemistry this present day: lipid dynamics and lipid-protein interactions, protein translocation and insertion, intracellular site visitors aud protein constitution and folding. the academics mentioned those subject matters ranging from a number of disciplines, together with biochemistry, cellphone biology, genetics, and biophysics. This wayan interdisciplinary and intensely inte~sting view on organic membrane platforms was once acquired. in the beginning an intensive evaluate of -mainly biophysical -techniques that are used to review dynamic methods in membranes used to be awarded. subtle ways resembling ESR and NMR were utilized succesfully to solve info of particular lipid-protein interactions. x­ ray research offers exact structural info of numerous proteins and the prospective implications for protein capabilities. info acquired this fashion is complemented through experiences on mechanisms and kinetics of protein folding. The latter details is vital while discussing protein translocation and insertion: proces:;es within which folding and unfolding play crucial roles. large perception used to be provided within the complex equipment of phospholipid biosynthesis. specifically, the appliance of subtle genetic innovations has allowed a greater knowing of the mechanisms regulating the bogus equipment and exact experiences on quite a few mutants, missing a number of of the fundamental enzymes, have ended in the start of a bL!:

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DETERMINATION OF TRANSBILAYER MOVEMENT OF PHOSPHOLIPIDS IN ERYTHROCYTES. ns - LTP plus PC I CHOLEST. p~ AND ·SM Lyso - PCI PEl PS AND CERAMIDE (from SM) '1= ~ Fig. 2. Principle of an experiment to determine the rate of trans bilayer movement of phospholipids in the erythrocyte membrane. In addition to the PC containing vesicles, indicated in the figure, it is possible to use any radioactive phospholipid in the donor vesicle system. Abbreviations; Hb, haemoglobin; PLEP, phospholipid exchange protein.

Coli. J BioI Chern 263 :7131-7153 Sparrow CP, Raetz CRH (1985) Purification and properties of the membrane-bound CDP-diglyceride synthetase of Escherichia coli. J BioI Chern 260:12084-12091 Vanden Boom T, Cronan, Jr. JE (1989) Genetics and regulation of bacterial lipid metabolism. Annu Rev Microbiol 43:317-343 Van der Goot FG, Didat N, Pattus F, Dowhan W, Letellier L (1993) Role of acidic lipids in the translocation and channel activity of colicins A and N in Escherichia coli. Eur J Biochem 213:217-221 Vasilenko I, de Kruijff B, Verkleij AJ (1982) Polymorphic phase behavior of cardiolipin from bovine heart and from B a c i II u s subtilis as detected by 31 P-NMR and freeze-fracture.

An important prerequisite for a better understanding of the role of sphingolipids in yeast is the knowledge about their subcellular distribution, the single steps of the biosynthetic pathway, and the routes and mechanisms of subcellular transport. In order to quantitate the small amounts of inositol-containing sphingolipids in yeast organelle membranes we developed a sensitive assay procedure. Yeast secretory mutants were used to study the interplay between biosynthesis and intracellular transport of sphingolipids, and the secretory pathway of proteins.

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