MFV3D Book Archive > Modern > Download Modern Methods of Plant Analysis / Moderne Methoden der by Dr. phil. H. F. Linskens, M. V. Tracey M. A., Dr. Ulrich PDF

Download Modern Methods of Plant Analysis / Moderne Methoden der by Dr. phil. H. F. Linskens, M. V. Tracey M. A., Dr. Ulrich PDF

By Dr. phil. H. F. Linskens, M. V. Tracey M. A., Dr. Ulrich Beiss, Dr. Fay Bendall, Fil. lic. Walter Björk, Professor Dr. F. Bohlmann, Hans G. Boman, Dr. sc. nat. Richard Braun, Dr. W. Heinen, cand. chem. Manfred Hesse, Dr. Eduard Hofmann, Dr. J. R. Hudson,

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Mn and Hg catalyze cysteine oxidation, but are ineffective in the case of glutathione. Mixtures of metals have been noted to act both synergistically and antagonistically It is clear therefore that one important factor in procedures designed to extract thiols quantitatively from tissues is the capacity of the extracting solution to immobilize the traces of metals invariably present. The desirability of using sulfosalicylic acid solutions to remove soluble thiols arises from the fact that this acid is an excellent agent for binding iron, and to some extent, copper.

2. Standardkurven zur Auswertung der Si- und P-Bestimmung (vg!. Text). Abstand von je 1 min) 4 ml einer 2%igen NaF-Losung, die vollauf geniigt, urn auch groBere Phosphatmengen vollstiindig zu unterdriicken. ) zusetzen. Nach dem Auffiillen mit Wasser zum Endvolumen (25 ml) wird photometrisch bei 660 mp, und zwar 45 min nach Zusatz der Reduktionslosung, ~o ----+-----j-------f--r--r--JT-----: wiederum in Abstiinden von je 1 min gemessen. Ergeben sich nach der Reduktion zu intensive Farbintensitiiten, die eine Messung nicht mehr ermoglichen, so kann mit Wasser verdiinnt werden (bis zum 10 fa chen Volumen).

Thioctic acid is essential to oxidative decarboxylation of pyruvate and has been considered to be an important controlling mechanism in the dark reactions of photosynthesis. Coenzyme A functions in acetylation reactions and is therefore important in fat, carbohydrate and protein metabolism. Thiamine, which along with thioctic acid, is essential to oxidative decarboxylation may also exist in sulfhydryl form. Thus, gross analysis of soluble thiol pools does not mirror the concentration of these important constituents.

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