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By A M Gewirtz

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Nishikura K. A short primer on RNAi: RNA-directed RNA polymerase acts as a key catalyst. Cell 2001; 107(4): 415–418. 29. Sharp PA. RNAi and double-strand RNA. Genes Dev 1999; 13(2):139–141. 30. Hutvagner G, McLachlan J, Pasquinelli AE, Balint E, Tuschl T, Zamore PD. A cellular function for the RNA-interference enzyme Dicer in the maturation of the let-7 small temporal RNA. Science 2001; 293(5531):834–838. 31. Ketting RF, et al. Dicer functions in RNA interference and in synthesis of small RNA involved in developmental timing in C.

J Pharm Sci 1997; 86(4): 438–442. 59. Laktionov PP, Dazard JE, Vives E, et al. Characterisation of membrane oligonucleotidebinding proteins and oligonucleotide uptake in keratinocytes. Nucleic Acids Res 1999; 27(11):2315–2324. Chapter 3 / Targeted Genome Modification II B ASIC METHODOLOGY 25 26 Part II / Basic Methodology Chapter 3 / Targeted Genome Modification 3 27 Targeted Genome Modification Via Triple Helix Formation Faye A. Rogers, PhD and Peter M. Glazer, MD, PhD CONTENTS INTRODUCTION TRIPLEX-FORMING OLIGONUCLEOTIDES TARGETED MUTAGENESIS TARGETED RECOMBINATION PEPTIDE NUCLEIC ACIDS LIMITATIONS AND FUTURE CHALLENGES REFERENCES 1.

Moser HE, Dervan PB. Sequence-specific cleavage of double helical DNA by triple helix formation. Science 1987; 238:645–50. 10. Lee JS, Johnson DA, Morgan AR. Complexes formed by (pyrimidine)n . (purine)n DNAs on lowering the pH are three-stranded. Nucleic Acids Res 1979; 6:3073–91. 11. Povsic TJ, Dervan PB. Triple helix formation by oligonucleotides on DNA extended to the physiological range. J Am Chem Soc 1989; 111:3059–3061. 12. Lacroix L, Lacoste J, Reddoch JF, et al. Triplex formation by oligonucleotides containing 5(1-propynyl)-2'-deoxyuridine: decreased magnesium dependence and improved intracellular gene targeting.

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