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Download On the spherically symmetrical statical field in Ensteins by Wiener N., Vallarta M.S. PDF

By Wiener N., Vallarta M.S.

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An alternative option would be two-photon FCS. The obvious next step will be to use FCS to investigate the interactions of the antenna proteins with luciferase. Such an interacting system is then composed of a natural reporter group. 28]. In the latter experiments the flavoprotein was trapped in a rigid matrix and the fluorescence photons of FAD of the immobilized enzyme were monitored. The fluorescence showed stochastic blinking behavior during enzymatic turnover as FAD is reduced by the substrate cholesterol.

30]. The dependence of the rate of re-association on the concentration of two strands was measured by FCB. 1) with Q corrects for the quantum yield difference of a free dye and a bound dye. 32]. 34]. Therefore, fluorescence emitted from tetramethylrhodamine residues is quenched in probes where the dye is located, such that the secondary structure of the 28 Z. F6ldes-Papp and M. Kinjo probe brings the guanosine nucleotide into the vicinity of the dye. Obviously, this effect is independent of the nucleotide length of dye-oligonucleotide or dye-DNA, but in fact it depends on the base sequence and on the number of atoms by which the dye is linked to the probe.

B The behavior of the fluctuations of the molecule number in two color crosscorrelation measurements analyzed under the effect of n = 11 green and m = 2 red labels covalently incorporated into the two color target: signal amplification of the target sequence. 16]. 57]. However, the "signal" amplification of a target sequence led to two color target molecules together with target sequences in one color. Post peR purification of the reaction mixes was necessary. The target yield was much lower in comparison with the "target" amplification via 5'-fiuorescently tagged primers.

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